Wednesday, February 26, 2014

From Gene to Protein: A Historical Perspective Protein vs. DNA

How did scientists discover the source of heritable genetic information?

Before we begin the discussion, let's review the structure of proteins.
Watch the video from education portal about the four levels of protein structure

Why might Rosalind Franklin's X-ray diffraction photograph have been interpreted as an alpha secondary structure?

Griffith's experiments (1928)
Go through the animation in Concept 17 (A Gene is Made from DNA) at DNA from the Beginning (dnaftb.org)
How did Griffith's discoveries help identify the source of genetic information?  Why were Griffith's "transforming factors" different from proteins?

Hershey-Chase Experiments (1952)
The results of Griffith's experiments began a quest among scientists to identify his "transforming factor". 
Watch the animation on the Hershey-Chase Experiment
What are the elements that make up a nucleic acid vs. a protein?
Why did Hershey and Chase use radioactive sulfer and phosphorus in their experiments?
Is DNA or protein the genetic material of a phage T2?
What evidence does this experiment give you to support this idea?
DNA Extraction 
How can DNA, a submicroscopic molecule, be visualized by the naked eye?
What must be done to extract and isolate DNA from human cheek cells?
What can we conclude about the chemical nature of DNA through isolation techniques?

Materials provided:
centrifuge tubes, disposable cups, sports drink, ethyl acohol, pipettes, cell lysis (detergent) solution, meat tenderizer (enzyme)

How will you use the above materials to extract DNA from your cheek cells?




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